How to get Stoned in five simple steps

the story of Uri Ace a Kid Knee Stone Mason

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208620/
Recent advances on the mechanisms of kidney stone formation (Review); Zhu Wang, Ying Zhang, Jianwen Zhang, Qiong Deng, and Hui Liang, Int J Mol Med. 2021 Aug; 48(2): 149. Published online 2021 Jun 11. doi: 10.3892/ijmm.2021.4982

Kidney stone disease is one of the oldest diseases known to medicine; however, the mechanisms of stone formation and development remain largely unclear.

Well, there’s an admission that gets me and Our Lady of Sardinia on the War Path. From the time that the first cave man or cave woman or cave fluid had a stitch in their side until now THEY HAVE NO IDEA HOW, WHY, OR WHAT TO DO ABOUT IT!
This is called: Modern Medicine?
Sounds more like a Three Stooges skit:

Over the past decades, a variety of theories and strategies have been developed and utilized in the surgical management of kidney stones, as a result of recent technological advances. Observations from the authors and other research groups suggest that there are five entirely different main mechanisms for kidney stone formation.

• Urinary supersaturation and crystallization are the driving force for intrarenal crystal precipitation.

• Randall's plaques are recognized as the origin of calcium oxalate stone formation.

• Sex hormones may be key players in the development of nephrolithiasis and may thus be potential targets for new drugs to suppress kidney stone formation.

• The microbiome, including urease-producing bacteria, nanobacteria and intestinal microbiota, is likely to have a profound effect on urological health, both positive and negative, owing to its metabolic output and other contributions.

• Lastly, the immune response, and particularly macrophage differentiation, play crucial roles in renal calcium oxalate crystal formation.

I’m a know-alot not a know-itall so I had to look up Randall’s plaques and, no, it’s not an award given to that guy from the Odd Couple…

Most of the short answer returns to a search were nonsense so we have to go for the best answer:
https://pubmed.ncbi.nlm.nih.gov › 26816774

The role of Randall plaques on kidney stone formation - PubMed

26816774 PMCID: PMC4708577 DOI: 10.3978/j.issn.2223-4683.2014.07.03 Abstract Randall's plaque is microscopically a plaque of calcium deposited in the interstitial tissue of the renal papilla. These plaques are thought to serve as a nidus for urinary stone formation.

Aren’t you glad you have a Farm Boy to break it all down for you?
Think of the cells as glass bricks with jelly inbetween so that fluid can go from brick to brick. A Randall plaque is like SOLID MORTAR between the bricks in a place where it should never be that then sets up for fresh mortar to be made ON THE OUTSIDE OF THE BRICKS.

See? Why couldn’t THEY say that? interstitial… papilla… nidus… Why I auttta rip our your esophagus! SLAP!

Butt, let me tell you why we’re even here:
The reasons listed above can go in to the soup-pot of why stones form but the major factors are:

• pH inside the tubules

• dehydration

• nucleation on microbes

The article above emphasizes oxalate stones which are part of the larger list of stones that precipitate when then urinary pH is too basic/alkaline. In this case consume large amounts of acid (NOT LSD YOU HIPPIES!) will suppress the minerals from falling out of solution. What no one covers is that Uric Acid stones precipitate out when the urine is too acidic in which case you NEED baking soda and suchlike to keep the urinary pH basic/alkaline to suppress the urates from precipitating.

I often wonder how many people following the advice of moronic gurus to take baking soda ‘for health’ when they had no demonstrable health problems to begin with will ultimately end up in the hospital for gout of uric acid kidney stones from bad advice?

Dehydration can be physical from sweating too much in the hot sun or a winter parka in the arctic. If you are in a particularly dry place ‘insensible losses’ can rob you of moisture without you even knowing about it.
Then, there’s the role of alcohol and tannins that can dry you out like a desert anywhere in your body but if it happens in the kidney tubules then that alone concentrates the minerals that would have been in solution to precipitate them out.

Hulda Clark said that Staph and Strep nucleate kidney stones.
That’s why we’re here today. I went looking for a Strep connection to stone formation and found that lists of 5 other things that they are ‘just discovering’ turns you into a stone mason.

• The microbiome, including urease-producing bacteria, nanobacteria and intestinal microbiota, is likely to have a profound effect on urological health, both positive and negative, owing to its metabolic output and other contributions.

Nanobacteria were supposedly found in Low Earth Orbit. They were blamed for the AstroNOTS developing kidney stones after space flights. I guess space ain’t sterile and if you went for Walkies outside the capsule they you brought the buggers back into the cabin. Not to worry, it’s not some exterrestrial plague - what goes up must come down so Bartonella (cat scratch fever - the real kind not Ted Nugent’s song), and Brucella (the nucleus of which was supposedly crystalized and rained down on us via chemtrails to cause AIDS), and Agrobacteria (used to make GMO crops) are quite ubiquitous and Terrestrial… although on writing this in the Jordanian Say It Like It Is And There Is No Debate fashion - it SEEMS like they are invaders from Outer Space!

Nanobacteria form calcium armor to protect themselves. This then makes us wonder if the Randall’s Plaque shouldn’t have been lumped in with this bullet point because that interstitial infiltration could have just been Bartonella from lyme setting up that mortar between the bricks that lived in the stones that Jack Built.

Through Hulda Clark I was aware of urease producing bacteria causing such horrors as histamine fits and that these buggers require Nickel to do their trick. Which is why many women gave up all of their jewelry in 2008 when they heard me decry the metallic shackles and handcuffs as Hertzian antennae to trap frequencies and harm your body. How many people wearing gold wedding rings or engagement rings with a semiconductor diamond mounted on it on their THYROID MERIDIAN finger have… thyroid problems?

But now I had to find a list of urease producing buggers:

https://www.nature.com/articles/1931106b0
PAYWALL this is all you’ll get:
The enzyme urease occurs in a wide variety of tissues in man, namely the gastric mucosa, liver, kidney, erythrocytes, etc., as well as in bacteria, yeasts, moulds, plants and molluscs. Sumner was the first chemist to obtain urease in crystalline form and showed that it was a protein of the globulin type with an isoelectric point of five. All species of the genus Proteus and many other genera of bacteria contain urease. The urease content of P. vulgaris is very high, and the rate of ammonia production of these organisms during growth has been used as a method of differentiating Proteus from other bacterial genera.

Without further inquiry we can lump lyme in with these critters because they are notorious for dumping ammonia. Make no mistake and don’t be deceived: Modern Fakery is OBSESSED with bacteria. But as you see above even Moulds (if you’re british) or Molds (if your not) make urease. And Hulda Clark said that worms are notorious for flooding the host with ammonia. Which is very curious why we have to scrub our toilets with cleansers if it’s already there?

But I wanted a list!

Even if you’re not a Lab Head, you might find this interesting to read the entire article:
https://microbeonline.com/urease-test-principle-procedure-interpretation-and-urease-positive-organsims/

Microorganisms Tested

• A. The urea test is part of the battery of tests to identify the following.

  • Gram-negative enteric pathogens, including Yersinia spp.

  • Fastidious Gram-negative rods—Brucella, H. pylori, and Pasteurella

  • Gram-positive rods—Corynebacterium and Rhodococcus spp.

  • Yeasts—Cryptococcus spp.

• B. Directly, this test is performed on gastric biopsy samples to detect the presence of H. pylori

Result and Interpretation 

Organisms that hydrolyze urea rapidly (Proteus spp., Morganella morganii, and some Providencia stuartii strains) will produce strong positive reactions within 1 or 6 hours of incubation;  delayed positive organisms (e.g. Klebsiella spp and Enterobacter species ) will produce weak positive reactions in the slant in 6 hours of incubation which will be intense during further incubation. The culture medium will remain a yellowish color if the organism is urease negative e.g. Escherichia coli. 

Diagnostic utility of Urease test

1. Urease test can be used as part of the identification of several genera and species of Enterobacteriaceae including Proteus and Klebsiella. It is also useful to identify Cryptococcus species, Brucella, Helicobacter pylori.

2. Urease test helps for the identification of Proteus species (urease positive) and to differentiate it from other non-lactose fermenting members of the Enterobacteriaceae family.

3. Urease test is used for the presumptive evidence of the presence of Helicobacter pylori in tissue biopsy material. This is done by placing a portion of crushed tissue biopsy material directly into urease broth. A positive urease test is considered the presence of Helicobacter pylori. Commercially available urease agar kits are also available.

4. Rapid urease test is can be used to differentiate between the yeasts, Candida albicans, and Cryptococcus neoformans. Presumptive identification of C. neoformans may be based on rapid urease production, whereas Candida albicans do not.

5. Urea breath test: A common noninvasive test to detect Helicobacter pylori also based on urease activity. This is a highly sensitive and specific test.

Name of urease positive organisms

1. Proteeae (Proteus spp., Morganella morganii, and some Providencia stuartii strains)

2. Cryptococcus spp

3. Corynebacterium spp

4. Helicobacter pylori

5. Brucella spp

Note: Mnemonics to remember urease positive organisms: PUNCH (Similar mnemonic for oxidase- positive organism is PVNCH)

• P: Proteus

• U: Ureaplasma

• N: Nocardia

• C: Cryptococcus neoformans/Corynebacterium spp

• H: Helicobacter pylori

I won’t make a joke about Punch & Yahoody puppet shows…

Butt did you notice that unless you are a farm boy that inhales Things Biological all day long that their mneumonic (that’s like pneumonia of the mind) was GROSSLY SIMPLIFIED AND UNFORGIVEABLY INCOMPLETE BASED ON THEIR OWN WRITING?

Proteus

Klebsiella Club Silly Yuh

[Enterobacter that contains BOTH proteus and klebsiella]

Cryptococcus yeast

BRUCELLA

H. pylori

Corynebacterium = diphtheria

YERSINIA

Actinomycetota is the phyla for Nocardia so where the hell did they pull that out of when it wasn't even mentioned until the memory reminder for idiot children pretending to be doctors? I mean: YERSINIA IS THE BLACK DEATH PLAGUE THAT STILL HAPPENS TO BE CARRIED BY RODENTS SO IF YOU GRADUATED KNOWING PUNCH WITHOUT JUDY THEN YOUR PATIEN MIGHT JUST DIE FOR YOUR INSTITUTIONALLY IMPREGNATED IGNORNACE!

Mycoplasmatota is the phyla for Ureaplasma that they just threw in there casually.

We have confirmation, then that Brucella the Nanobacteria also makes urease therefore predisposing Uric Acid stone formation due to the high acidity of ammonia at the kidney tubles requiring that baking soda to calm things down. We were thrown a curve with the Black Death, and given a few more non-strep (our original inquiry) and non-staph (I wish I had a staff to do this work) players in this game of masonry.

P = proteus
U= Ureaplasma
N= Nocardia
K= Klebsiella
E= Enterobacter
D= Diphtheria = Corynebacterium

B= Brucella
Y= Yersinia

H= Helicobacter pylori
C= Cyrptococcus

No one should leave their house without a Farm Boy. Otherwise serious harm is on the horizon. You might get PUNKED.

I highly recommend the article below as it addresses many of the points that I made above in a thorough way so, although I skim articles these days, I might add it to my list of only 5 articles in 23 years that were actually science thus making this #6.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208620/
Mechanisms of Stone Formation; Vishal N Ratkalkar, and Jack G Kleinman; Clin Rev Bone Miner Metab. 2011 Dec; 9(3-4): 187–197. doi: 10.1007/s12018-011-9104-8

[don’t forget that many articles offer a download of the pdf form at the right of the article header. this is a very good paper.]

There are at least four types of inhibitors in urine: small organic anions such as citrate, small inorganic anions such as pyrophosphates, multivalent metallic cations such as magnesium, or macromolecules such as osteopontin and Tamm-Horsfall protein. The table is a listing of substances generally considered to inhibit stone formation and the stone formation process they appear to inhibit.

Now you either have to be an MD researcher, a pharmacist, or a farmboy geek or a baker to know that pyrophosphate outside of the baking powder (so as not to be corn fused with baking soda) is the anionic pair of THIAMINE which really puts a tailspin on this pole-dancer since all of your Anti-Oxalate religious nuts will say that you MUST have B1 = thiamine to fight the Evil Oxalic Acid. But is it really the Thiamine or the Beriberi that we all now have? or was it something as simple as the B1 being a PYROPHOSHATE = DIPHOSPHATE = COCARBOXYLASE DONOR?

I mean: speaking purely from a farmboy geek point of view where I am uniquely qualified to be able to discern the smell of chicken, from pig, from cow feces, this smells more like a SETUP but the Military Food Industrial Complex by POISONING the food supply with the potentially carcinogenic Thiamine Mononitrate INSTEAD OF A PYROPHOSPHATE thus robbing people of the anion that could protect them against stones with the Disaster Capitalism - never let a good food disaster go to waist - scenario of also KEEPING PEEPLE BEETEN DOWN BY THIAMINE DEFICIENCY IF THEY ARE GENETICALLY INCAPABLE OF MAKING THE PYROPHOSPHATE ACTIVE FORM FROM MONONITRATE OR HCL SALTS!

I’m sorry kids, I spend WAY TOO MUCH time trying to cram as much knowledge into my mud pellet to the point of losing sleep only to oversleep to catchup with my ketchup to find that the single DEFICIENCY that might be HEALTHY to mankind is a total ABSENCE OF GOVERN MENTE.

All of this and all I was trying to find out is if Strep produced urease!

Just so you know when I search and type at the same time an article like this can take a couple of hours. When I search before I post that kind of work can take a couple of days. One of the Rate Limiting Factors as a chemist would say is whether or not the A.I. is playing with me like a cat with a mouse in a bathtub. I searched AGAIN for strep producing urease and got a Sir Prize:

https://www.nature.com/articles/s41598-020-65107-9
Inhibition of urease activity by different compounds provides insight into the modulation and association of bacterial nickel import and ureolysis; Simon Svane, Jens Jakob Sigurdarson, Friedrich Finkenwirth, Thomas Eitinger & Henrik Karring; Scientific Reports volume 10, Article number: 8503 (2020)

Abstract

The nickel-dependent urease enzyme is responsible for the hydrolysis of urea to ammonia and carbon dioxide. A number of bacteria produce urease (ureolytic bacteria) and are associated with various infectious diseases

and ammonia emissions from agriculture.

We report the first comprehensive comparison of the inhibition of urease activity by compounds analysed under the same conditions. Thus, 71 commercially available compounds were screened for their anti-ureolytic properties against both the ureolytic bacterium Klebsiella pneumoniae and purified jack bean urease. Of the tested compounds, 30 showed more than 25% inhibition of the ureolytic activity of Klebsiella pneumoniae or jack bean urease, and among these, carbon disulfide, N-phenylmaleimide, diethylenetriaminepentaacetic acid, sodium pyrrolidinedithiocarbamate, 1,2,4-butanetricarboxylic acid, tannic acid, and gallic acid have not previously been reported to possess anti-ureolytic properties. The diverse effects of metal ion chelators on ureolysis were investigated using a cellular nickel uptake assay. Ethylenediaminetetraacetic acid (EDTA) and dimethylglyoxime (DMG) clearly reduced the nickel import and ureolytic activity of cells,

oxalic acid stimulated nickel import but reduced the ureolytic activity of cells,

1,2,4-butanetricarboxylic acid strongly stimulated nickel import and slightly increased the ureolytic activity of cells, while L-cysteine had no effect on nickel import but efficiently reduced the ureolytic activity of cells.

---

I’m really hoping that our Little Red Hen: SandMan can spend some quality time with this material because I consider him the foremost unbiased expert on the question of oxalates out there. I had a: AREYOUFREAKINKIDDINGME moment with this:

oxalic acid stimulated nickel import but reduced the ureolytic activity of cells

so a little farmboyanization is required: Oxalic Acid, Public Enema #1 of the AlterNOTive universe allowed microbes to take up the nickel that leads to the production of the Urea-splitting enzyme inside the bugs BUT IT DID NOT ALLOW THEM TO SPLIT THE UREA… much.

This is ALWAYS the dialectic that I have fought for 23 years without a break:
Oxalate is Bad for you - Oxalate is Good for you.

My response after 23 years has been: Neither.
Oxalic Acid or its precipitated salt: Oxalate is neither good nor bad in the NeitherLands. The ONLY thing that makes it harmful to human physiology is the DAMAGE THAT THEY DID TO OUR CYTOPLAMSIC, MITOCHONDRIAL AND GENETIC FUNCTIONS.

Until Googlevil takes it down:

In collaboration with our Little Red Hen Sandwich Lady and our dearly departed Grannie Annie, this work should have rocketed me to internet and international fame and a non-club member No Bell prize. As it stands the first video got 561 views in 3 years meaning that the entire world will die in ignorance because they are all NPC followers of the Pie Eyed Piper that will hypnotize the Chillens into the lake with his flute. My compliments to those 561 that did watch it or the series.

Lettuce move on said the Bowels:

Think the A.I. is pissing with me when I SPECIFICALLY SEARCHED:
does strep produce urease
Not to worry since Hulda said that Staph and Strep nucleate kidney stones:

https://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1007538
Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection; Chunyi Zhou,Fatema Bhinderwala,McKenzie K. Lehman,Vinai C. Thomas,Sujata S. Chaudhari,Kelsey J. Yamada,Kirk W. Foster,Robert Powers,Tammy Kielian,Paul D. Fey; Version 2; Published: January 4, 2019; https://doi.org/10.1371/journal.ppat.1007538

Abstract

Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity.

Golden (aurum) Staph is one of those hospital-acquired Flesh Eating buggers IF and ONLY IF they are infected with a bacteriophage virus (butt we all know that viruses don’t exist so don’t feel stress about having a bad hair day when you’re in the hospital or you might get the Golden Flesh Eater).

Urease, an enzyme that generates NH3 and CO2 from urea, is key to pH homeostasis in bacterial pathogens under acidic stress and nitrogen limitation.

See? SEE? EVEN BACTERIA ARE UNDER STRESS!!!!
Wait… that was physiochemical stress, they weren’t having a Bad Hair Day…

However, the function of urease in S. aureus niche colonization and nitrogen metabolism has not been extensively studied.

Sardinia has had a totally separate geological history from that of the rest of Italy, where several cycles of volcanic activity occurred, the last of which ended at the beginning of the Pleistocene, but currently hosts only permanently extinct volcanoes.

Well… that is until Our Lady of Sardinia reads that nonsene in the article and I’m sure that she’s coming out of extinction to go all pumice, ash, and lava on the idiots that vomit the SAME THING OVER AND OVER FOR 5783 YEARS!

We doan no noffin’ bout studeyin no Aureus!

We discovered that urease is essential for pH homeostasis and viability in urea-rich environments under weak acid stress. The regulation of urease transcription by CcpA, Agr, and CodY was identified in this study, implying a complex network that controls urease expression in response to changes in metabolic flux. In addition, it was determined that the endogenous urea derived from arginine is not a significant contributor to the intracellular nitrogen pool in non-acidic conditions. Furthermore, we found that during a murine chronic renal infection, urease facilitates S. aureus persistence by promoting bacterial fitness in the low-pH, urea-rich kidney. Overall, our study establishes that urease in S. aureus is not only a primary component of the acid response network but also an important factor required for persistent murine renal infections.

And they called Hulda Clark a nut and the FTC chased her out of North America into Mexico! We don’t know why you make kidney stones. We don’t know why you have chronic kidney infections. Scientists (who the hell are they?) are studying it. We need more funding.

For 5 millennia? I want my money back.

So why wasn’t Staph listed in the PUNKED BY CT list?
Lettuce keep going. Remember how I already covered Crohns?

Crohn's on the M.A.P.

Changing gut bacteria in Crohn’s disease; NIH RESEARCH MATTERS; December 5, 2017

Researchers discovered that an enzyme called urease altered the composition of bacteria in the guts of mice and led to an inflammatory bowel disease.

The study suggests that urease may be a potential target for treating inflammatory bowel diseases such as Crohn’s disease and ulcerative colitis.

Crohn’s disease is a chronic disease that causes inflammation and irritation in the digestive tract. This can lead to severe pain, diarrhea, and poor absorption of nutrients, which can stunt a child’s growth in severe cases. The disease most often begins gradually and can become worse over time.

Doctors aren’t sure what causes Crohn’s disease.

Mount Sardinia and Mount Grain Ghetto just blew!

In the days of court Soothsayers if you made a bad prediction you were Ex Eye Cute Ted. I wonder how many permanent cures there would be if doctors were held to the same standard?

Some experts suggest that changes in the gut microbiome—the community of microbes, like bacteria and viruses, living in the gut—may play a role.

I know life is distracting… Did Kim Kardashion just burp?
But please keep the two statements above in mind for later.

Gut bacteria metabolize nitrogen in our intestine to produce the amino acids and enzymes they need to flourish. Certain types of bacteria taking over the gut may contribute to inflammatory bowel disease, including Crohn’s disease.

To investigate the relationship between bacterial nitrogen metabolism and inflammatory bowel diseases, a team led by Dr. Gary D. Wu at the University of Pennsylvania analyzed gut microbes and amino acids in patients with Crohn’s disease. The research was supported in part by NIH’s National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) and National Institute of General Medical Sciences (NIGMS). Results were published in Science Translational Medicine on November 15, 2017.

The team's previous work showed increased activity in genes associated with nitrogen metabolism in stool samples taken from 90 children with Crohn’s disease compared with samples from 26 healthy children. In the current study, the researchers found higher levels of amino acids and their derivatives in stool samples from the people with Crohn’s disease. These were correlated with high levels of a group of gut bacteria called Proteobacteria.

Some bacteria in the colon use an enzyme called urease to produce ammonia. The ammonia serves as a major source of nitrogen to feed their growth. The researchers tracked nitrogen in mice with normal gut bacteria, depleted gut bacteria, or guts that were depleted and recolonized with bacteria that have low levels of urease. They found that the enzyme allows gut bacteria to produce ammonia that is then used to make amino acids.

The scientists wiped out gut microbiomes in mice

and then introduced Escherichia coli (a type of Proteobacteria) that were genetically engineered to produce either high or low levels of urease.

High levels of urease caused Proteobacteria to flourish in the mouse guts. Low levels of urease led to healthier gut microbiomes.

Now I axed you to remember because of two things:
The culture medium will remain a yellowish color if the organism is urease negative e.g. Escherichia coli. 

Remember that from the top of my Stack? E. coli DOES NOT PRODUCE UREA! So what did Tony Fauciland (NIH= Not Intended for Health) do? THEY MADE A BIOLOGICAL WEAPON THAT CAN BE DEPLOYED VIA A MOUSE VECTOR!

They call it research. They call it genetic modification.
Say it like it is - then there is no debate:
The NIH made a biological weapon in contravention of UN Treaty but EVERYONE turns a blind eye to it because they renamed it using Black Magick Spellwork to be called something that it is not!

In mice prone to colitis, the high levels of urease worsened colitis. These mice lost more weight and had more inflammation than mice given E. coli that produced low levels of urease.

Using a technique similar to that used to wipe out the gut microbiome in mice, the team showed that they could reduce gut bacteria in five people. This suggests a way to alter gut bacteria in people that may lead to a healthier microbiome.

“Because it’s a single enzyme that is involved in this process, it might be a targetable solution,” says Wu. “The idea would be that we could ‘engineer’ the composition of the microbiota in some way that lacks this particular [enzyme].”

NOTHING that they have ‘engineered’ so far has benefitted mankind so why haven’t they all been burnt at the stake for open sorcery?

The group is now investigating treatments for Crohn’s disease based on these findings in clinical studies.

—Tianna Hicklin, Ph.D.

I threw this in there because it was part of my search returns for Strep producing urease but it goes to show that not only does the urease allow for persistent infection but it ERODES the tissue at the focus of infection. Infected bowels = eroded bowels. Infected kidneys = you finish this sentence…

When consulting the A.I. Oracle: does strep produce urease
It replies with Wicked Peed On Us.

Now in the past I used to discount that NSA construct out of hand until a saw a post on how RAIN was NUCLEATED by pseudomonas syringae BACTERIA. It went on to say that P. syringae was one of the most studied genomes in bacteria. I sat up and took notice. When the article went on to say that they had been DEPLOYING IT FOR OVER 100 YEARS IN AGRICULTURE TO ATTEMPT TO FORCE RAIN then I knew we were pretty much done as a species and that Wicked Peed On Us was a legitimate source for information because they were cataloging and HIDING IN PLAIN SITE things that only an adept at their level or an Anti-A.I. Little Red Hen could see the significance of.

Let’s see what the Wicked have to say about Urease:
A 1984 study focusing on urease from jack bean found that the active site contains a pair of nickel centers. In vitro activation also has been achieved with manganese and cobalt in place of nickel.

Manganese brings us into the World of Lyme.

All bacterial ureases are solely cytoplasmic, except for those in Helicobacter pylori, which along with its cytoplasmic activity, has external activity with host cells. In contrast, all plant ureases are cytoplasmic.

This is a reverse Las Vegas meme where what happens inside H. pylori DOES NOT STAY INSIDE H. PYLORI.

Action in pathogenesis

Bacterial ureases are often the mode of pathogenesis for many medical conditions. They are associated with hepatic encephalopathy / Hepatic coma, infection stones, and peptic ulceration.

Infection stones

Infection induced urinary stones are a mixture of struvite (MgNH4PO4•6H2O) and carbonate apatite [Ca10(PO4)6•CO3]. These polyvalent ions are soluble but become insoluble when ammonia is produced from microbial urease during urea hydrolysis, as this increases the surrounding environments pH from roughly 6.5 to 9. The resultant alkalinization results in stone crystallization. In humans the microbial urease, Proteus mirabilis, is the most common in infection induced urinary stones.

Iddn’t this a kick in the kideys? I spent HOURS putting together this stack seeking out solid reserach on the topic yet all the time I only needed to go to the NSA vomit site to get what I needed to prove Hulda I-Had-To-Run-To-Mexico Clark was RIGHT ALL THE TIME. Well, OF COARSE, she had to run to Mexico - SHE WAS RIGHT!

There is complex chemistry here that we as a group have to figure out because to a degree it is counter-intuitive:
The function of urease is to break down urea into ammonia. Ammonia is highly toxic to human cells is highly basic/alkaline = pH 11, but the Ammonium ion formed in the presence of water is said to be weakly acidic but I can’t find a pH value for it but the alternative view is that it is so acidic at the kidney tubules that it can burn them so you need a buffer in the form of baking soda that is pH 9.

Sorry kids

Urease in hepatic encephalopathy / hepatic coma

Studies have shown that Helicobacter pylori along with cirrhosis of the liver cause hepatic encephalopathy and hepatic coma. Helicobacter pylori release microbial ureases into the stomach. The urease hydrolyzes urea to produce ammonia and carbonic acid. As the bacteria are localized to the stomach ammonia produced is readily taken up by the circulatory system from the gastric lumen. This results in elevated ammonia levels in the blood, a condition known as hyperammonemia; eradication of Helicobacter pylori show marked decreases in ammonia levels.

Urease in peptic ulcers

Helicobacter pylori is also the cause of peptic ulcers with its manifestation in 55–68% reported cases. This was confirmed by decreased ulcer bleeding and ulcer reoccurrence after eradication of the pathogen. In the stomach there is an increase in pH of the mucosal lining as a result of urea hydrolysis, which prevents movement of hydrogen ions between gastric glands and gastric lumen. In addition, the high ammonia concentrations have an effect on intercellular tight junctions increasing permeability and also disrupting the gastric mucous membrane of the stomach.

All of this time spent to find the LIST of organisms through other sources that AVOIDED THE INCLUSION OF STAPH AND STREP when all the time Wicked Peed On Us had it in their nearly complete lineup:
Urease-positive pathogens include:

Proteus mirabilis and Proteus vulgaris

Ureaplasma urealyticum, a relative of Mycoplasma spp.

Nocardia

Corynebacterium urealyticum

Cryptococcus spp., an opportunistic fungus

Helicobacter pylori

Certain Enteric bacteria including Proteus spp., Klebsiella spp., Morganella, Providencia, and possibly Serratia spp.

Brucella

Staphylococcus saprophyticus

Staphylococcus aureus

BUT I WANTED STREP!!!! [insert toddler meltdown here]

Before we take a STREP in the right direction let’s see what the Wicked tell us might HELP!

Inhibitors

A wide range of urease inhibitors of different structural families are known. Inhibition of urease is not only of interest to agriculture, but also to medicine as pathogens like H. pylori produce urease as a survival mechanism. Known structural classes of inhibitors include:

Analogues of urea, the strongest being thioureas like 1-(4-chlorophenyl)-3-palmitoylthiourea.

• Phosphoramidates, the most commonly used in agriculture (see above).

• Hydroquinone and quinones. In medicine, the most interesting are quinolones, already a class of widely used antibiotics.

• Some plant metabolites are also urease inhibitors, an example being allicin. These have potential both as environmentally-friendly fertilizer additives and natural drugs.

Aren’t ya’ll glade that I’m skinny and light weight so that you can stuff me under your arm and carry me out into public for the times when you need to know what to buy to protect you against the things that they done did to ya’ll’s?

Thiourea as found in silver polish is just damned poison for your thyroid.

But then if you had a memory or the knowledge in said memory then Thiosulfates are the bigger family under with the thiosulfate of thiourea finds a home. So then you would remember that the Thiosulfates as found in Cruciferate vegetables like brocolli, Brussel sprouts [YUK!], cabbage, kohlrabi (NOT Kohl Nidre), turnips, kale, mustard, and the pure poison: Cauliflower - avoid at all costs! well… except the cost of my book:

and I’m going to make stretch here by guess: Horseradish are all consider cancer protecting ANTI-FREAKINGOXIDANTS that no one tells you (because they want to sell you the NEXT remedy) are also goitergenic just like the silver polish. You never get something for nothing down here. You can reduce the thiosulfates by cooking with steam or boiling, but now I’m wondering if the folks that claim miracles cures from eating this stuff raw probably had moderately strong thyroids but were overrrun with urease-forming buggers????

I don’t know about you but I’m exhausted and we haven’t even gotten to the main feature. It’s not me. I hate those competition shows that say: And now the winner is…. coming up right after this break.

The A.I. literally held out to the end of the review cycle to give me these two legs-up.

https://pubmed.ncbi.nlm.nih.gov/8550211/

Streptococcus salivarius urease: genetic and biochemical characterization and expression in a dental plaque streptococcus; Y Y Chen, K A Clancy, R A Burne; PMID: 8550211 PMCID: PMC173805 DOI: 10.1128/iai.64.2.585-592.1996

Abstract

The hydrolysis of urea by urease enzyme of oral bacteria is believed to have a major impact on oral microbial ecology and to be intimately involved in oral health and diseases. To begin to understand the biochemistry and genetics of oral ureolysis, a study of the urease of Streptococcus salivarius, a highly ureolytic organism which is present in large numbers on the soft tissues of the oral cavity, has been initiated. By using as a probe a 0.6-kpb internal fragment of the S. salivarius 57.I ureC gene, two clones from subgenomic libraries of S. salivarius 57.I in an Escherichia coli plasmid vector were identified. Nucleotide sequence analysis revealed the presence of one partial and six complete open reading frames which were most homologous to ureIAB-CEFGD of other ureolytic bacteria. Plasmid clones were generated to construct a complete gene cluster and used to transform E. coli and Streptococcus gordonii DL1, a nonureolytic, dental plaque microorganism. The recombinant organisms expressed high levels of urease activity when the growth medium was supplemented with NiCl2. The urease enzyme was purified from E. coli, and its biochemical properties were compared with those of the urease produced by S. salivarius and those of the urease produced by S. gordonii carrying the plasmid-borne ure genes. In all cases, the enzyme had a Km of 3.5 to 4.1 mM, a pH optimum near 7.0, and a temperature optimum near 60 degrees C. S. gordonii carrying the urease genes was then demonstrated to have a significant capacity to temper glycolytic acidification in vitro in the presence of concentrations of urea commonly found in the oral cavity. The ability to genetically engineer plaque bacteria that can modulate environmental pH through ureolysis will open the way to using recombinant ureolytic organisms to test hypotheses regarding the role of oral ureolysis in dental caries, calculus formation, and periodontal diseases. Such recombinant organisms may eventually prove useful for controlling dental caries by replacement therapy.

Continuum Thinking will allow you to just ease into the idea that most microbes favor an infection focus. This can be the sinuses, tonsils, mouth, gallbladder, appendix, prostate or uterus. Anything downstream of those will be recipients of secondary colonization. So it is easy to comprehend that if you have an oral infection that it will populate the tonsils and sinuses so ANYTHING downstream of that location will be at risk - which means the whole body. This is not to say that it can’t or won’t crawl into your Mud Pellet as well.

Also thinking in Continuum will allow the crossover of the notion of the release of the concept that Treponema denticola is an oral infection of the genus that SYPHILIS comes from so I don’t even allow that kind of Black Magicks Sleight of Word to trick me into thinking that it is anything but MOUTH SYPHILIS and that we all have it just like Lida Mattman said. THEREFORE, this Streptococcus salivarius (wuddn’t he a famous violin maker?) is just a PLEOMORPHIC FORM OF STREP AS WELL that just happens to colonize via adaptation the mouth instead of the skin. But talk about adaptation: They readily acknowlege that Group A Beta Hemolytic strep can go from the skin to Necrotizing Fasciitis (flesh eating disease that is not flesh eating it is an over-reacting immune response that burns up the host tissue) to moving indoors to give rheumatic fever (rheumatoid arthritis in joints) or glomerulonephritis in the kidneys.

So is it just syphilis when it is in the No-No’s but something else when it is in the mouth? Then is it just strep throat when it is in the throat but something different when it is in the kidneys?

Our Little Red Hen Western Fence Lizard brought up the question of just what the hell Algae is? Back in my day they lumped it in it’s own Kingdom and said that it wasn’t a plant, but cyanobacteria can be considered a bacteria… When I looked it up these Narrative-re-writing mongrels said that it actually spans SIX KINGDOMS from plant to bacteria to animal, etc. The Corn Clusion that Western Fence Lizard brought me to is that it is PLEOMORPHIC! So it takes on the form adapted to the environment that it is in. All of my schooling from 1973 Four Word went down the Toity Hole.

Streptococcus salivarius? Don’t think so.

Dental caries? Any relation to Jim Carey?
Because the article was just as stupidly funny. They GMOed E. coli (a shit bacteria) to test a theory of mouth bacteria (not really a stretched base on the shit that I’ve heard come out of most ‘experts’ mouths) so now even if strep and its urease (see how I can keep on topic after about ten pages of mind-numbing diatribe?) were NOT a factor in oral disease the new BIOWEAPON in the form of E. coli and its urease chemical warfare agent WILL BE. They don’t make these things to NOT realease them on the Project Paperclip Stalag inmates!

What is dental plaque but biofilm?
Heed well the warning of either Sapi or Macdonald to: LEAVE THE BIOFILM ALONE! if you go all enthusiatic New Age AlterNut Guru follower then you will try to break up the biofilm for ‘health’ while unleashing Pandoras Pox on your body that has no capacity to deal with what is coming out of the armored ooze. And I ain’t talkin’ Amore.

So with this pile of data it appears that kidney stones start in the mouth!

The strep enters the host via the mouth, sets up focal infections anywhere in the oral cavity, tonsils, nasopharynx, the populates downward. It appears that if you want to prevent kidney stones you have to address it in the mouth. But how does it GET THERE?

Ever eat FREAKIN’ YOGHURT?

Who put the Hurt in Yoghurt?

https://pubmed.ncbi.nlm.nih.gov/17993384/
Urease production by Streptococcus thermophilus; Food Microbiol. 2008; Teresa Zotta 1, Annamaria Ricciardi, Rocco Rossano, Eugenio Parente; Feb;25(1):113-9. doi: 10.1016/j.fm.2007.07.001. Epub 2007 Jul 25. PMID: 17993384

Abstract

In order to identify potential alternative sources of urease for the removal of urea from alcoholic beverages, 205 strains of lactic acid bacteria belonging to 27 different species were screened for urease production. Only Streptococcus thermophilus produced urease. Cell permeabilization with toluene allowed to increase activity significantly. Optimal pH for urease activity in whole and permeabilized cells and of cell free extracts differed slightly, but was in the range 6.0-7.0. Significant activity was retained at pH 3.0 and 8.0, and, for cell free extracts, at pH 4.0 in the presence of ethanol. Urease production was evaluated in fermentations with pH control (5.25-6.5) and without pH control. Very little urease was produced in absence of urea, which at 5g/l slowed growth significantly in fermentations without pH control, but prevented a decrease in pH below 5.1 and resulted in higher final biomass. Optimal pH for growth was between 6.0 and 6.5 but specific urease activity was higher for fermentations at low pH at the beginning of the exponential phase. However, a higher total urease activity was obtained at pH 6.0 and 6.5 because of higher biomass. Potential technological applications of urease production by S. thermophilus are discussed.

But Oh Great Sage of Continuum! You were talking about how your Yog Hurt, why dost thou bringeth in the beereth into the discussion? Thou dost confuseth us to the highest order!

Because my Dear Readers: Thou must have the mind of a pharmacist, a chemist, a biologist, and a foodist to harmonize all of the knowledge of the universe to arrive at The Answer! That an totally not have a life…

https://www.sciencedirect.com › science › article › pii › S1756464617304267
Streptococcus thermophilus: From yogurt starter to a new promising ...
Streptococcus thermophilus is a Gram positive bacterium widely used in dairy fermentations for the production of yogurt and cheese. In contrast with other lactic acid bacteria, the probiotic status of S. thermophilus remains still questioned.

Do you see why we can’t cohabit wherever this is with these creatures?
THEY PUT UREASE FORMING BACTERIA IN THE SPOILED MILK THAT YOU EAT FOR

HEALTH!

Then they have the Hoots Puh to shrug and say: Vell, ve don’t even know if it’s good for goy!

AND YOU WONDER WHY YOU HAVE FREAKIN’ KIDNEY STONES?

I’ve eaten yoghurt before way deep in my youth and HATED IT, but that meant that I was polluted before I made the adult decision to eat only a vegan diet and ferment my own foods without DUPONT telling me what THEY were going to put in their WEAPONS OF MASS DISTRIBUTION.

https://corporationnationradioarchives.files.wordpress.com/2015/05/show357_april27.mp3

Listen to me very carefully my Children of Continuum. I am brash, I am flippant, I’m a lot of things but I’m SERIOUS that the radioshow linked above the the BEST BROADCAST IN HUMAN HISTORY. It set the course and understanding for where we are today. Dupont started out as a munitions maker.

https://libertarianinstitute.org/articles/merchants-of-death-origin-of-the-military-industrial-complex/

just because the went from munitions to paint to yoghurt to the DISCOVERERS OF THE CRISPR WEAPON doesn’t mean that they EVER stopped making weapons!

I don’t know about you, but I’ve been doing this all day since I got up. I’m tired.

Eat garlic.

Break urease.

Repell vampyres.

Be well.